Introduction of YACs into mamm.cells
ANDREI.POPOV at afrc.ac.uk
Thu Jan 12 13:36:50 EST 1995
we introduce large YACs into murine ES cells by yeast protoplast
fusion.I have not seen anything published on the introduction
of YACs by protoplast fusion into human cells.
The limit for microinjection is currently about
600kb, and there is only one guy in this world who
made it. I cannot see any advantage of lipofection
over protoplast fusion.
andrei.popov at bbsrc.ac.uk
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