Mitochondrial Isolation

[fcrump at indyvax.iupui.edu]

I am curious if anyone working on mitochondrial bioenergetics knows if the
isolation procedure of Johnson and Lardy preserves the outer mitochondrial
membrane in its native state.  I have conflicting evidence in 2 journals 
but they are quite old.  I would rather not have to go through a series of
swelling experiments with hypotonic solutions and monoamine oxidase marker
enzyme experiments if I dont have to.