droos at sas.upenn.edu
Sun Jan 15 21:25:34 EST 1995
Sigurdur Einar Vilhelmsson wrote:
> I recently saw an ad in Nature regarding a detectionkit for mycoplasma from
> Boehringer Mannheim. This kit is based on a combination of PCR and ELISA.
> Since I work in a small lab that doesn't have the necessary equipment for
> analysing the ELISA, I was wondering if anyone knew of a mycoplasma
> detection kit based on PCR only. I would be very greatful for any
> information on this, either E-mail or a follow-up.
Anyone with access to a fluorescence microscope with suitable
illumination/filter sets can easily assay for mycoplasma contamination by
staining with Hoechst 33258. For methods see T.R.Chen (1977)
Exp.Cell.Research 104:255-262. It's cheap and easy ... no need for an
-- D.S.Roos, Dep't Biology, Univ. Pennsylvania (droos at sas.upenn.edu)
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