RNA lifetime in tissue samples

Sigurdur Einar Vilhelmsson sev at rhi.hi.is
Thu Jan 19 11:06:09 EST 1995


Hi everyone.

Here at the lab, we are planning to start isolating total RNA from either
tissue samples or blood samples.  The tissue route is preferred, but as
the samples are sent to us from hospitals, they are not completely fresh.
They are stored in RPMI 1640 medium, cont. pen/strept, and are sometimes
up to 36 hrs. old.
What I would like to know is, if it is reasonable to try to isolate RNA
from samples that old, because of RNA breakdown.  The RNA will mostly be
used for cDNA transcripts.

S.E. Vilhelmsson
sev at rhi.hi.is




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