Microsome prep from cultured cells: methods?
DSMT45A at prodigy.com
Thu Jan 19 21:24:32 EST 1995
My lab is currently isolating microsomes from cultured cells,
specifically an enterocyte line, but the procedure we use has been used
for HepG2 cells in the past as well. Unfortunately I do not have the
procedure here at home this evening. I will bring it home tomorrow night
and post it if I can. Basically, you just harvest a large number of cells
(by scraping) and put them through several cycles of freeze-thaw (liquid
N2/37oC). After that it's just a matter of going through a standard
microsome preparation by differential sedimentation.
B.SALISBURY DSMT45A at prodigy.com
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