Microsome prep from cultured cells: methods?

[Brian Salisbury]

My lab is currently isolating microsomes from cultured cells, 
specifically an enterocyte line, but the procedure we use has been used 
for HepG2 cells in the past as well. Unfortunately I do not have the 
procedure here at home this evening. I will bring it home tomorrow night 
and post it if I can. Basically, you just harvest a large number of cells 
(by scraping) and put them through several cycles of freeze-thaw (liquid 
N2/37oC). After that it's just a matter of going through a standard 
microsome preparation by differential sedimentation.
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  B.SALISBURY  DSMT45A at prodigy.com