Phosphorothioate oligo questions
krg at utmdacc.mda.uth.tmc.edu
Fri Jan 20 10:55:41 EST 1995
I have a couple of questions reguarding anti-sense phosphorothioated
oligonucleotides. I will be using 20-mers in tissue culture. First, is it
necessary for the entire probe to be phosphorothioated? Second, when adding
oligo to tissue culture flask is there one way to combine better than
others??? i.e. should I combine oligo and lipofectin prior to adding to cells??
Thanks in advance.
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