staining oil droplets

HOBBSD hobbsd.hobbsd-jii at afrc.ac.uk
Tue Jan 24 03:57:11 EST 1995


I need a method for the rapid screening of cell lines producing
triacylglycerol ( oilbodies ).

We have tried staining colonies with Nile red ( Greenspan et al., 1985 ) but
appear to have a problem with inspecific staining ( possibly to hydrophobic
domains of some proteins and/or other lipids).

If anybody has used the above method with some success, have they any
suggestions? Or alternatively, can anyone propose a different stain or method
to resolve the problem in hand.

Thanks in anticipation of your reply.

Doug. 



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