staining oil droplets
hobbsd.hobbsd-jii at afrc.ac.uk
Tue Jan 24 03:57:11 EST 1995
I need a method for the rapid screening of cell lines producing
triacylglycerol ( oilbodies ).
We have tried staining colonies with Nile red ( Greenspan et al., 1985 ) but
appear to have a problem with inspecific staining ( possibly to hydrophobic
domains of some proteins and/or other lipids).
If anybody has used the above method with some success, have they any
suggestions? Or alternatively, can anyone propose a different stain or method
to resolve the problem in hand.
Thanks in anticipation of your reply.
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