staining oil droplets

Brian Salisbury DSMT45A at prodigy.com
Wed Jan 25 22:14:25 EST 1995


Try this technique: Fix cells with 10% formalin (neutral-buffered). Stain 
oil droplets using 0.5% oil red O in 70% triethylphosphate (balance: 
water). You might need to do a time-course to optimize staining but I 
would start with staining the fixed cells for 5 minutes first. If you 
want to put a cover slip on the preparation I suggest using warmed 
glycerol-gelatin.




More information about the Cellbiol mailing list