TGF-beta bioassay: interference
etorreel at vub.ac.be
Tue Jan 31 13:03:27 EST 1995
I have problems to measure active/total TGF-beta with the classical bioassay,
based on growth inhibition of the Mv1Lu cell line. My samples (tumor cell
conditioned medium) seem to contain (a) factor(s), that stimulate(s) the
proliferation of the Mv1Lu cells (and also alters their morphology).
Does anyone recognise this problem ?
Does anyone know about particular factors which are mitogenic for the Mv1Lu
cells (or morphogenic) ?
Is there an alternative assay which distinguishes between active and latent
Any suggestion is wellcome
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