Mammalian Electroporation!>!>

Simon Lovestone spjusil at ucl.ac.uk
Fri Jul 14 04:52:46 EST 1995


Graham Dellaire <popa0206 at PO-Box.McGill.CA> wrote:
>HAs anyone out there obtained an electroporation frequency
>better than 10-5 transformants/no# cells transformed???
>
>If you have I would like to find out if your cell type and
>what make of Gene Pulser you have etc.... 
>
>I am having a hell of a time breaking the 10-5 barrier murine L-fibroblasts.
>
>
>Graham
>
>

Whilst on the subject, we too get very low frequency using human 
fibroblasts. Any good electroporation protocols out there ??

=================================================================
Simon Lovestone             |     s.lovestone at ucl.ac.uk
                            |
Institute of Psychiatry     |




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