spjusil at ucl.ac.uk
Fri Jul 14 04:52:46 EST 1995
Graham Dellaire <popa0206 at PO-Box.McGill.CA> wrote:
>HAs anyone out there obtained an electroporation frequency
>better than 10-5 transformants/no# cells transformed???
>If you have I would like to find out if your cell type and
>what make of Gene Pulser you have etc....
>I am having a hell of a time breaking the 10-5 barrier murine L-fibroblasts.
Whilst on the subject, we too get very low frequency using human
fibroblasts. Any good electroporation protocols out there ??
Simon Lovestone | s.lovestone at ucl.ac.uk
Institute of Psychiatry |
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