Mammalian Electroporation!>!>

tyla stiles tyla at ix.netcom.com
Fri Jul 21 18:27:40 EST 1995


In <1995Jul14.095246.24736 at ucl.ac.uk> Simon Lovestone
<spjusil at ucl.ac.uk> writes: 
>
>Graham Dellaire <popa0206 at PO-Box.McGill.CA> wrote:
>>HAs anyone out there obtained an electroporation frequency
>>better than 10-5 transformants/no# cells transformed???
>>
>>If you have I would like to find out if your cell type and
>>what make of Gene Pulser you have etc.... 
>>
>>I am having a hell of a time breaking the 10-5 barrier murine
L-fibroblasts.
>>
>>
>>Graham
>>
>>
>
>Whilst on the subject, we too get very low frequency using human 
>fibroblasts. Any good electroporation protocols out there ??
>
>=================================================================
>Simon Lovestone             |     s.lovestone at ucl.ac.uk
>                            |
>Institute of Psychiatry     |
>




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