tyla at ix.netcom.com
Fri Jul 21 18:27:40 EST 1995
In <1995Jul14.095246.24736 at ucl.ac.uk> Simon Lovestone
<spjusil at ucl.ac.uk> writes:
>Graham Dellaire <popa0206 at PO-Box.McGill.CA> wrote:
>>HAs anyone out there obtained an electroporation frequency
>>better than 10-5 transformants/no# cells transformed???
>>If you have I would like to find out if your cell type and
>>what make of Gene Pulser you have etc....
>>I am having a hell of a time breaking the 10-5 barrier murine
>Whilst on the subject, we too get very low frequency using human
>fibroblasts. Any good electroporation protocols out there ??
>Simon Lovestone | s.lovestone at ucl.ac.uk
>Institute of Psychiatry |
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