Microinjection

[Louis-Eric Trudeau]

jbelyea at ACPUB.DUKE.EDU (Jennifer Belyea) wrote:
>Hello netters!
>Does anybody have a good reference on microinjecting expression plasmids
>into the nuclei of cells, especially PC12 cells?



You  would probably need an Eppendorf microinjection system.
For a ref on microinjection in PC12 cells (antibodies not plasmids however) look for papers published by the lab of R.H. Scheller
in the last five years (look for the paper on injection of synaptotagmin antibodies).

Good Luck

Louis-Eric Trudeau, Ph.D.
Iowa State University
Letrud at iastate.edu