S1 nuclease mutation detection kit?

Dan Johnson djohnson at biost1.thi.tmc.edu
Fri Jun 23 09:23:20 EST 1995


In article <lab_sakano-2206951527380001 at lsa6mac31.berkeley.edu>,
lab_sakano at maillink.berkeley.edu (Linda Kingsbury) wrote:

> Does anybody know who sells a kit for detecting mutations using S1
> nuclease digestion?....  


   One source to try is:

   Ambion Inc.
   2130 Woodward St.
   Austin, TX 78744-1832
   Ph. 512-445-6979
   FAX 512-445-7139

   I used their kit with some modifications, and it was fair.  I would not
suggest their asymmetric PCR method for amplifying a probe, in that the
probe I got was WAY too cold for any assays.  Otherwise, its a kit form of
the protocol listed in the Red Book for the most part.  It is convenient,
but I am unsure it is worth paying for, compared to just following the Red
Book protocol.

   Good luck.

   Dan Johnson
   Texas Heart Institute



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