David L. Bradbury
dbradbur at umabnet.ab.umd.edu
Tue Jun 27 13:52:37 EST 1995
I am currently working with an androgen independent prostate
cancer cell line. Using the Oncor ApopTag kit, I have gotten beautiful
pictures of cells undergoing apoptosis, however, there are a few
phenomenas that I notice that I can't explain- namely:
1. Apoptosis is supposed to be marked by cell shrinkage whereas
my cells (which underwent ionizing irradiation) are markedly larger than
the control cells.
2. In the positive controls and cells that undergo a lot of
apoptosis, I notice a lot of cells have FITC label only on the membrane
creating a "ring" around these cells. Is this indicative of early apoptosis?
(the cells have been fixed)
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