Transfecting pirmary cell lines

Dermot Cox dcox at medmicro.uct.ac.za
Wed Mar 1 16:13:10 EST 1995


We are having problems  transfecting primaries derived from foetal bovine 
testes with plasmid DNA using the DOTAP transfection kit from 
Boeringher-Mannhiem.  Has any one had similar problems with this kit or could 
recommend a better method.

Thanks 
Dermot Cox
Dept. Med. Micro.
UCT Medical School 
Cape Town 
South Africa




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