Transfecting pirmary cell lines

Dermot Cox dcox at
Wed Mar 1 16:13:10 EST 1995

We are having problems  transfecting primaries derived from foetal bovine 
testes with plasmid DNA using the DOTAP transfection kit from 
Boeringher-Mannhiem.  Has any one had similar problems with this kit or could 
recommend a better method.

Dermot Cox
Dept. Med. Micro.
UCT Medical School 
Cape Town 
South Africa

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