BrDU proliferation assay
benoit.arveiler at u-bordeaux2.fr
Thu Mar 2 09:14:09 EST 1995
In article <1995Feb23.173459.7706 at ucsvc.ucs.unimelb.edu.au>,
chris at austin.unimelb.edu.au wrote:
> Has anyone had experience with the BrDU labelling assay
> for cell proliferation? I have attempted to use it in
> a manner analagous to the continuous labelling of cells
> with tritiated thymidine. This a simple method giving
> much information of cell cycle time etc. However,
> rather than getting a simple increase in labelling over
> time with an eventual plateau when all cells are
> labelled, I obtained a sine curve with two peaks, the
> second less than the first. I would be glad of any
> explanations (or even half convincing fictions!).
> Thanks in advance,
> Chris Bradley
BrdU may be toxic for some cells; thus in the first time you kill the
sensible one, and you observe a decrease of signal, then you have an
increasing incorporation in a fewer number of cell (smaller peak).
To get a fait idea about the sensibility of your cells just number them
during the BrdU treatment using the blue trypan to check viability
Avoid benveniste-like sine curves in the future!
and good luck...
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