cloning PCR products

[Paul M. Coussens]

PCR products are not blunt ended, they have a terminal A at the 
3' end.  To efficiently clone PCR products, you either have to 
treat with T4 polymerase or use a T-Vector, such as Promega's 
pGEM-T.  Other T-vectors are available from Stratagene and 
Clontech.  

-- 
Dr. Paul M. Coussens
Molecular Virology Laboratory
Michigan State University
cousssens at pilot.msu.edu