cloning PCR products

Paul M. Coussens 71742.1127 at CompuServe.COM
Fri Mar 3 17:15:39 EST 1995


PCR products are not blunt ended, they have a terminal A at the 
3' end.  To efficiently clone PCR products, you either have to 
treat with T4 polymerase or use a T-Vector, such as Promega's 
pGEM-T.  Other T-vectors are available from Stratagene and 
Clontech.  

-- 
Dr. Paul M. Coussens
Molecular Virology Laboratory
Michigan State University
cousssens at pilot.msu.edu



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