cloning PCR products
deroche1 at pilot.msu.edu
Fri Mar 3 15:55:16 EST 1995
In article <9502027941.AA794185234 at warren.med.harvard.edu>,
xia at WARREN.MED.HARVARD.EDU wrote:
> Hi, my netter friend! I'm trying to cloning a PCR product by
> blunt-end ligation. Is there any suggestion for efficient
> cloning? I have to clone my PCR products into a EcoRV blunt
> site, should I treat PCR product before ligation?
Synthetic oligonucleotides usually lack 5' phosphates. Therefore, you
should treat with T4 kinase either before of after amplification.
More information about the Cellbiol