cloning PCR products

Amy DeRocher deroche1 at pilot.msu.edu
Fri Mar 3 15:55:16 EST 1995


In article <9502027941.AA794185234 at warren.med.harvard.edu>,
xia at WARREN.MED.HARVARD.EDU wrote:

> 
>           Hi, my netter friend! I'm trying to cloning a PCR product by
>           blunt-end ligation. Is there any suggestion for efficient
>           cloning? I have to clone my PCR products into a EcoRV blunt
>           site, should I treat PCR product before ligation?
Synthetic oligonucleotides usually lack 5' phosphates.  Therefore, you
should treat with T4 kinase either before of after amplification.
Amy DeRocher



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