ELISA for human IgG
inger.natvig at rh.uio.no
Fri Mar 10 05:01:14 EST 1995
We are currently working to establish ELISA for human IgG in biological fluids.
We have tried coating with both monoclonal and one polyclonal anti human IgG.
However, we have problems with high background using the polyclonal antibody
and poor coating efficency or high background using the monoclonals.
My question is whether anyone could recommend a suitable combination of
coating antibody and secondary reagents that could be available to us.
Thanks in advance
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