ELISA for human IgG

Inger Natvig inger.natvig at rh.uio.no
Fri Mar 10 05:01:14 EST 1995


We are currently working to establish ELISA for human IgG in biological fluids. 
We have tried coating with both monoclonal and one polyclonal anti human IgG.  
However, we have problems with high background using the polyclonal antibody 
and poor coating efficency or high background using the monoclonals. 
My question is whether anyone could recommend a suitable combination of 
coating antibody and secondary reagents that could be available to us. 

 		
				
Thanks in advance
				
Inger Natvig  



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