Vacuoles/Empty spaces in Endothelial cells

Jason at UofSask jir130 at
Sun Mar 19 03:49:56 EST 1995

I'm currently culturing bovine brain endothelial cells.  I've noticed 
that in some of the cells there appears to be what looks like vacuoles or 
empty spaces (ranging from small ones to some that are about 1/4 the size 
of the cell) in the cytoplasm of the cells, usually near the nucleus.  
The cells don't seem to be dying as they are able to reach confluence.  
Does anyone have any ideas as to what may be causing the formation of 
these vacuoles/spaces and how can it be eliminated from the culture?  
Could the cause be the serum (calf serum) or the basic fibroblast 
growth factor (human, recombinant) being used?

TIA for any help/advice or comments given.

Jason Ram

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