non-radioactive assays for cell proliferation

Laszlo Radvanyi radvanyi at oci.utoronto.ca
Sat Mar 18 13:57:47 EST 1995


     A few non-radioactive assays are available on the market.  The most 
popular one is the MTT assay.  Essentially, the assay measures the 
activity of mitochondrial dehydrogenases in viable cells.  The more 
cells, the more MTT reduction in a culture.  The assays can be performed 
in 96-well plates and read using an ELISA reader.  Kits are available 
from Sigma.  Alamar Blue dye that can be used in a similar 
reductive-based assay and is also available on the market.

     Have you tried counting cell with a haemocytometer.  Its 
old-fashioned, but believe me it works! 

Laszlo  



More information about the Cellbiol mailing list