non-radioactive assays for cell proliferation
radvanyi at oci.utoronto.ca
Sat Mar 18 13:57:47 EST 1995
A few non-radioactive assays are available on the market. The most
popular one is the MTT assay. Essentially, the assay measures the
activity of mitochondrial dehydrogenases in viable cells. The more
cells, the more MTT reduction in a culture. The assays can be performed
in 96-well plates and read using an ELISA reader. Kits are available
from Sigma. Alamar Blue dye that can be used in a similar
reductive-based assay and is also available on the market.
Have you tried counting cell with a haemocytometer. Its
old-fashioned, but believe me it works!
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