non-radioactive cell growth assay
michael.micksche at univie.ac.at
Mon Mar 20 04:37:51 EST 1995
In article <3ka4pk$52r at usenet.INS.CWRU.Edu>, ecg at po.CWRU.Edu (Edward C. Goodwin) says:
> If anyone has any suggestions for non-radioactive cell growth assays I would
>greatly appreciate it if you would drop me a line. Our lab has been studying growth
>arrest upon expresssion of certain viral proteins and uses tritium incorporation as an
>assay for DNA synthesis. I seem to recall an add for some ELISA assay that would measure the
>same thing, so if anyone out there in cyberland has any experience with this, positive or
>negative, I'ld really like to hear about it.
> Thanks in Advance,
> Ed Goodwin
>Edward C. Goodwin PhD ecg at po.cwru.edu
>Department of Genetics, SHM I-166 ecg at po.cwru.edu@cunynm (bitnet)
>Yale University School of Medicine "Facts do not cease to exist
>333 Cedar Street because they are ignored."
A very good and accurate MTT-Assay Kit is available from Biomedica, Vienna, Austria.
The procedure is quick and simple, it encompasses only dissolving the substrate in a buffer,
pipetting into the wells of 96-plates, incubation (approx 2-3hrs) and measurement of the OD 450.
The regressioncurve between cellnumber/well and OD 450 is almost linear from 1000 to 5000 cells/well
(Test line: SK-MEL 28) and the coefficient of standarddeviation is approx. 5-10% .
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