Vacuoles/Empty spaces in Endothelial cells

[Washington dos Santos]

In article <Pine.OSF.3.91.950319022615.20687A-100000 at duke.usask.ca>, "Jason at UofSask" <jir130 at duke.usask.ca> says:
>
> vacuoles or 
>empty spaces (ranging from small ones to some that are about 1/4 the size 
>of the cell) in the cytoplasm of the cells, usually near the nucleus.  

Have you done any sort of staining for lipid (Sudam III) ?
Some toxic lesions leads to lipid accumulation. 
We had some problems with a batch of plasma derived serum, two yr ago.

Wluis