Methods of mammalian cell lysis

R.G. Chaudhari rmrrgc at leeds.ac.uk
Wed Mar 22 13:30:42 EST 1995


Does anyone have any ideas/protocols for lysing monolayer cells and cell 
nuclei without detergents. I am particularly interested in simple and swift 
methods that do not rely on high salt conditions/sonication as I am looking at 
potential protein complexes that are v.sensitive and labile-especially to 
detergents.

Cheers 
Ravindra Chaudhari.



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