Methods of mammalian cell lysis

Ian A. York york at
Thu Mar 23 15:09:46 EST 1995

>In article <rmrrgc.9.0012BAF2 at>, rmrrgc at (R.G. Chaudhari) says:
>Does anyone have any ideas/protocols for lysing monolayer cells and cell 
>nuclei without detergents.

	Have you looked into doucne homogenizers?  They worked for me.  I 
believe I used a protocol from Methods in Enzymology vol 182.  I just 
wanted cytoplasm, but I believe you can also get nuclei from this.

Ian York   (york at
Dana-Farber Cancer Institute, 44 Binney St., Boston MA 02115
Phone (617)-632-3921     Fax  (617)-632-2627

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