Methods of mammalian cell lysis

Ian A. York york at mbcrr.dfci.harvard.edu
Thu Mar 23 15:09:46 EST 1995


>In article <rmrrgc.9.0012BAF2 at leeds.ac.uk>, rmrrgc at leeds.ac.uk (R.G. Chaudhari) says:
>
>Does anyone have any ideas/protocols for lysing monolayer cells and cell 
>nuclei without detergents.

	Have you looked into doucne homogenizers?  They worked for me.  I 
believe I used a protocol from Methods in Enzymology vol 182.  I just 
wanted cytoplasm, but I believe you can also get nuclei from this.

Ian
-- 
Ian York   (york at mbcrr.harvard.edu)
Dana-Farber Cancer Institute, 44 Binney St., Boston MA 02115
Phone (617)-632-3921     Fax  (617)-632-2627




More information about the Cellbiol mailing list