Methods of mammalian cell lysis
Ian A. York
york at mbcrr.dfci.harvard.edu
Thu Mar 23 15:09:46 EST 1995
>In article <rmrrgc.9.0012BAF2 at leeds.ac.uk>, rmrrgc at leeds.ac.uk (R.G. Chaudhari) says:
>Does anyone have any ideas/protocols for lysing monolayer cells and cell
>nuclei without detergents.
Have you looked into doucne homogenizers? They worked for me. I
believe I used a protocol from Methods in Enzymology vol 182. I just
wanted cytoplasm, but I believe you can also get nuclei from this.
Ian York (york at mbcrr.harvard.edu)
Dana-Farber Cancer Institute, 44 Binney St., Boston MA 02115
Phone (617)-632-3921 Fax (617)-632-2627
More information about the Cellbiol