Methods of mammalian cell lysis

Jason at UofSask jir130 at duke.usask.ca
Thu Mar 23 15:26:14 EST 1995



On Wed, 22 Mar 1995, R.G. Chaudhari wrote:

> Does anyone have any ideas/protocols for lysing monolayer cells and cell 
> nuclei without detergents. I am particularly interested in simple and swift 
> methods that do not rely on high salt conditions/sonication as I am looking at 
> potential protein complexes that are v.sensitive and labile-especially to 
> detergents.
> 
> Cheers 
> Ravindra Chaudhari.
> 

I'm not sure if this will help you but I use a 20 mM NH4OH (in 
H20) solution to lyse and solubilize the cell monolayer of bovine 
corneal endothelial cells (as part of the denudation process leaving behind 
the ECM laid by these cells).  Perhaps you could adapt this method to 
your protocol.        

Jason Ram



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