Methods of mammalian cell lysis

Dave Bates dobates at ucdavis.edu
Thu Mar 23 14:31:51 EST 1995


In article <rmrrgc.9.0012BAF2 at leeds.ac.uk>
rmrrgc at leeds.ac.uk (R.G. Chaudhari) writes:

> Does anyone have any ideas/protocols for lysing monolayer cells and cell 
> nuclei without detergents. I am particularly interested in simple and swift 
> methods that do not rely on high salt conditions/sonication as I am looking at 
> potential protein complexes that are v.sensitive and labile-especially to 
> detergents.
> 
> Cheers 
> Ravindra Chaudhari.

Try putting them in water. Blows the cell membrane nicely. Don't know
what it does to the nuclei though.


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