Methods of mammalian cell lysis

[Dave Bates]

In article <rmrrgc.9.0012BAF2 at leeds.ac.uk>
rmrrgc at leeds.ac.uk (R.G. Chaudhari) writes:

> Does anyone have any ideas/protocols for lysing monolayer cells and cell 
> nuclei without detergents. I am particularly interested in simple and swift 
> methods that do not rely on high salt conditions/sonication as I am looking at 
> potential protein complexes that are v.sensitive and labile-especially to 
> detergents.
> 
> Cheers 
> Ravindra Chaudhari.

Try putting them in water. Blows the cell membrane nicely. Don't know
what it does to the nuclei though.


------------------------------------------------------------------------
-
Dave Bates PhD                                     Tel: 916 752-7081
Postdoctoral Researcher                            Fax: 916 758-2554 
Dept of Human Physiology                         email:
dobates at ucdavis.edu
University of California at Davis                drink: anything
Davis, California 95616, USA            No I don't have a sense of
humour 
                                                now buy me a beer      
------------------------------------------------------------------------
----