Resuscitating a Hybridoma line

David J. Meyer meyerdj at biovx1.biology.ucla.edu
Fri Mar 24 16:32:55 EST 1995


In article <3kumsc$nrj at mserv1.dl.ac.uk>, ANDREI POPOV
<ANDREI.POPOV at bbsrc.ac.uk> wrote:
(stuff deleted)
> Try  feeder cells (normally isogenic splenocytes work great)
(more stuff deleted)
I agree with trying feeder cells. Another option (one which gave me what I called the "Lazarus clone") is to use conditioned medium- I believe we used 0.2 micron sterile filtered medium from a Buffalo rat liver cell line. Thanks to Dr. Dwane Wylie for the original suggestion of trying this.
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David J. Meyer
Department of Biological Chemistry
University of California-Los Angeles
meyerdj at biovx1.biology.ucla.edu
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