3T3

[Dennis Goode]

Dr. Sharon Powell on 24 Mar 1995 06:50:44 -0800 wrote:
> 
>  -Can anyone out there suggest a simple method for getting 3T3 to 
> adhere to glass coverslips for immunofluorescence experiments?  I 
> have tried poly-lysine coating the coverslips but the cells still 
> tend to come off.  Thanks.
> -Sharon Powell
> Sharon K. Powell
> Assistant Research Scientist
> Department of Zoology
> University of Maryland, College Park
> 
Sharon,

A product called "Cell-Tak", which I believe is made from the glue 
that attaches clam muscles to the shell, is more effective than 
polylysine. Call 1-800-seaglue or walk over to my lab and I may be 
able to give you a sample.

-Dennis