Resuscitating a Hybridoma line

ANDREI POPOV ANDREI.POPOV at bbsrc.ac.uk
Sat Mar 25 14:57:33 EST 1995


> Try  feeder cells (normally isogenic splenocytes work great)
(more stuff deleted)
>I agree with trying feeder cells. Another option (one which gave me what I
>calle
>d the "Lazarus clone") is to use conditioned medium- I believe we used 0.2
>micro
>n sterile filtered medium from a Buffalo rat liver cell line. Thanks to Dr.
>Dwan
>e Wylie for the original suggestion of trying this.
--
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^

>David J. Meyer                                             

I have also tried to use conditioned medium.
The best was that from 5637 human bladder carcinoma cell line.
It also produces LIF as well as a bunch of other cytokines.

Andrei




More information about the Cellbiol mailing list