wierd cell culture contam

Susanne Gollin gollin at MED.PITT.EDU
Thu Mar 30 07:54:19 EST 1995

did you take the medium to your local microbiology lab to have it cultured?

Also, why are you using so many antibiotics in your culture medium?  You
may wish to use gentamicin, pen, strep, clindamicin, chloramphenicol,a nd
fungizone for a few days on primary cultures, but then, gentamicin should
be enough to keep most cultures clean.  Purists say that cultures should
contain no antibiotics.  We use an initial culture medium for biopsies,
since they are not usually manipulated under sterile conditions in the
Pathology lab and then use gentamicin for continued cultures.

just a few thoughts.

Susanne Gollin
Dept. of Human Genetics
University of Pittsburgh Graduate School of Public Health

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