Resuscitating a Hybridoma line

seppen at seppen at
Fri Mar 31 05:43:49 EST 1995

In Article <kirberg-2603951549460001 at>
kirberg at (Jorg Kirberg) writes:
>In article <D61BoM.930 at>, crm at (pogo) wrote:
>> In article <gustilo-2303951334480001 at>, 
>> gustilo at says:
>> >I'm trying to resuscitate a hybridoma line that we have. I usually grow
>> >the lines in DMEM HG, 10% FCS, HT, Non-Essential AA, Sodium Pyruvate,
>> >HEPES. The other lines grow well in this medium but there is one in
>> >particular that just won't grow.
>> >

You could try to add some old medium from cells which have grown confluent. I
use this trick always when i am subcloning cells.
Jurgen Seppen
The Netherlands 

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