Resuscitating a Hybridoma line

seppen at amc.uva.nl seppen at amc.uva.nl
Fri Mar 31 05:43:49 EST 1995


In Article <kirberg-2603951549460001 at seven.bii.ch>
kirberg at bii.ch (Jorg Kirberg) writes:
>In article <D61BoM.930 at dorite.use.com>, crm at iquest.net (pogo) wrote:
>> In article <gustilo-2303951334480001 at renal3.med.upenn.edu>, 
>> gustilo at pobox.upenn.edu says:
>
>> >I'm trying to resuscitate a hybridoma line that we have. I usually grow
>> >the lines in DMEM HG, 10% FCS, HT, Non-Essential AA, Sodium Pyruvate,
>> >HEPES. The other lines grow well in this medium but there is one in
>> >particular that just won't grow.
>> >
>

You could try to add some old medium from cells which have grown confluent. I
use this trick always when i am subcloning cells.
Greetings,
Jurgen Seppen
Amsterdam
The Netherlands 




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