Enzyme chimeras

FNINER fniner at aol.com
Thu Mar 30 17:55:12 EST 1995


Dear Colleagues:
I am interested in learning if anybody has attempted to create
protein/protein receptor/ligand  binding assays in which one of the
interacting components was a chimera consisting of any of the known
reporter enzymes such as alk. phosphatase, glucuronidase, glucose oxidase,
etc.  The advantage being the receptor-binding of the enzyme-tagged ligand
can be readily tested via its enzymatic activity without "extensive"
washing steps that might cause "some undesirable" dissociation of the
R/ligand complexes.  Appreciate any info. concerning the above.
fniner at aol.com



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