(none)

SMTPBIT1 at VTBIT.CC.VT.EDU SMTPBIT1 at VTBIT.CC.VT.EDU
Thu May 25 15:26:48 EST 1995


Hi, netters, maybe a dumb question, but here it goes anyway.
We want to do immunoprecipitation using protein A sepharose beads.
Protocols I've seen recommend microcentrifugation at 10-12,000 g
for 5-20 seconds. Is it out of the question using 5,000 g for whatever
time is necessary? The reason I ask is that i have a portable
Costar microfuge that goes up to 5,500 g maximum, and the
nearest centrifuge that goes up to 12,000 g is some 12 labs
away (I'm in Neurobiology, and this is anatomy/electrophysiology
land). Can I stick my portable microfuge in a fridge and work at
4 degrees and 5,500 g to do immunoprecipitation?
Thanks.

_____________
Rafael Linden
IBCCF-UFRJ
E-mail: rlinden at brlncc.bitnet
        rlinden at chagas.biof.ufrj.br
Phone: 55-21-290-6897 Fax: 55-21-280-8193



More information about the Cellbiol mailing list