BSA alternatives for immunoblocking?

Morrison Lab fergusb at microbio.lifesci.ucla.edu
Thu Nov 2 20:38:28 EST 1995



1 or 2 per cent milk powder in PBS ususlly does the trick - there are many
different ways but milk powder is a generally applicable one

Fergus
UCLA

In article <4760mb$fcg at news.cis.okstate.edu>, asb7223 at okway.okstate,edu wrote:

> Dear Colleagues
> 
> I am attempting to show the presence of sheep serum albumin (SSA) 
> in various tissues of parasitic ticks infesting sheep.  I have a
> polyclonal anti-BSA antibody and am using it for Western blotting (I 
> could not find an anti-SSA commercially available, but believe the 
> anti-BSA should cross-react).  My problem:  what can I use as a blocking
> agent - dried milk or BSA cannot be used.  I tried 3% pig skin gelatin for
> 45 min but this gave unsatisfactory blocking as all of my sample proteins
> lit up beautifully.
> 
> Does anyone have any ideas to alteratives regarding blocking proteins or
> different protocols??
> 
> Cheers
> 
> Alan S. Bowman



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