BSA alternatives

Daniel Lazard dlazard at netvision.net.il
Sat Nov 4 09:28:53 EST 1995


In article <478t5g$4sn at mserv1.dl.ac.uk>, Andrei Popov
<ANDREI.POPOV at bbsrc.ac.uk> wrote:

> > My problem:  what can I use as a blocking
> >agent - dried milk or BSA cannot be used.  I tried 3% pig skin gelatin for
> >45 min but this gave unsatisfactory blocking as all of my sample proteins
> >lit up beautifully.
> >
> >Does anyone have any ideas to alteratives regarding blo
> >cking proteins or different protocols??
> 
> Fish gelatin?
> Other makes of plates?
 
You could try a synthetic polymer like PVP (polyvinylpyrrolidone)

-- 
Dr. Daniel Lazard                          Tel: 03-9376280
Felsenstein Medical Research Center        Fax: 03-9211478
Beilinson Campus, Petah-Tikva 49100     e-mail:dlazard at shani.co.il
Israel



More information about the Cellbiol mailing list