dlazard at netvision.net.il
Sat Nov 4 09:28:53 EST 1995
In article <478t5g$4sn at mserv1.dl.ac.uk>, Andrei Popov
<ANDREI.POPOV at bbsrc.ac.uk> wrote:
> > My problem: what can I use as a blocking
> >agent - dried milk or BSA cannot be used. I tried 3% pig skin gelatin for
> >45 min but this gave unsatisfactory blocking as all of my sample proteins
> >lit up beautifully.
> >Does anyone have any ideas to alteratives regarding blo
> >cking proteins or different protocols??
> Fish gelatin?
> Other makes of plates?
You could try a synthetic polymer like PVP (polyvinylpyrrolidone)
Dr. Daniel Lazard Tel: 03-9376280
Felsenstein Medical Research Center Fax: 03-9211478
Beilinson Campus, Petah-Tikva 49100 e-mail:dlazard at shani.co.il
More information about the Cellbiol