BSA alternatives

Daniel Lazard dlazard at
Sat Nov 4 09:28:53 EST 1995

In article <478t5g$4sn at>, Andrei Popov
<ANDREI.POPOV at> wrote:

> > My problem:  what can I use as a blocking
> >agent - dried milk or BSA cannot be used.  I tried 3% pig skin gelatin for
> >45 min but this gave unsatisfactory blocking as all of my sample proteins
> >lit up beautifully.
> >
> >Does anyone have any ideas to alteratives regarding blo
> >cking proteins or different protocols??
> Fish gelatin?
> Other makes of plates?
You could try a synthetic polymer like PVP (polyvinylpyrrolidone)

Dr. Daniel Lazard                          Tel: 03-9376280
Felsenstein Medical Research Center        Fax: 03-9211478
Beilinson Campus, Petah-Tikva 49100     e-mail:dlazard at

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