BLOOD processing for DNA extraction

vid mohan-ram k9mhc at
Mon Nov 6 01:21:58 EST 1995

>I'm trying to determine what may be the best blood processing protocol for 
>future DNA extractions...<snip>

two good methods.....guftason et al analytical biochemistry, 165, 294-299, 
1987...this uses 'normal' organic extraction and the authors did a good 
set of controls using different anticoagulants etc and processed the 
samples at different times etc....

secondly lahira et al, Nucl. Acids Res. 19, p5444, a very nice 
protocol....NO ORGANIC handling which is nice..very quick and you dont need to 
pellet your cells before by sucrose lysis and salting out

hope these help

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