BLOOD processing for DNA extraction

vid mohan-ram k9mhc at rfhsm.ac.uk
Mon Nov 6 01:21:58 EST 1995


>I'm trying to determine what may be the best blood processing protocol for 
>future DNA extractions...<snip>

two good methods.....guftason et al analytical biochemistry, 165, 294-299, 
1987...this uses 'normal' organic extraction and the authors did a good 
set of controls using different anticoagulants etc and processed the 
samples at different times etc....

secondly lahira et al, Nucl. Acids Res. 19, p5444, 1991..is a very nice 
protocol....NO ORGANIC handling which is nice..very quick and you dont need to 
pellet your cells before hand....works by sucrose lysis and salting out

hope these help



More information about the Cellbiol mailing list