staining of apoptotic cells
Dra. Elisa Bal de Kier-Joffe
elisa at invrof.anmat.sld.ar
Mon Nov 6 14:46:34 EST 1995
We are using Acridine Orange (AO) to stain apoptotic cells.
The nuclei staining of apoptotic cells is very easily recognized when it is
in an advanced stage.
However when the process is early I observe only a brighter staining of
chromatin clumps, and a more marked limit in nuclei periphery. When this
happens, the cytoplasm shows a diffuse red staining which is also evident in
definite apoptotic cells.
In contrast normal alive cells only show a pale diffuse green staining of
the nuclei and cytoplasm and only a kind of red granule (RNA) in the
cytoplasm next to the nucleus.
I have not found any comments concerning this early diffuse intense red
staining of the cytoplasm with AO in apoptotic and preapoptotic cells.
Could you help me with any ideas, references, etc.?
Thanks a lot
(also you can reply to postmaster at invrof.anmat.sld.ar)
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