FBS trouble part2
iemery1 at cc.swarthmore.edu
Tue Nov 7 16:06:11 EST 1995
Thanks for all the helpful comments I received. Here are answers to
some of your questions,
The FBS lots I had trouble with were: 37N7052 and 32K2646.
The new bottle I received lot 30P2155 looks good.
The bugs I see (or I think I see) are visible on an inverted scope at
400X magnification but you have to concentrate to see the little dots
moving about. It helped when I would swirl the medium and I would get
a concentrated spot in the middle of the plate.
I took some contaminated media and passed it through a 0.2um filter
and after two days I saw the bugs again. So I figured it went through
the filter albeit inefficiently.
I did call Life Technologies and they said no one else had complained
but that they would look into it. I am expecting a call from them
I guess it could be small precipitate undergoing brownian motion.
This was, in fact, my first impression but I don't see this in the
freshly opened medium from the fridge only after an overnight in the
incubator and there would be so much of it! In another
lot, like I mentioned, I didn't see the bugs. I even asked a couple
of people in my department to come and take a look. It looked
biological to others too. Precipitate might still be the problem
and different batches behave differently on this regard.
Maybe I am imagining the whole thing but one thing I am sure of,
my project was working great until I started seeing this light cloudi
ness on my media that under 400X mag looked like tiny bugs to me. I
hope this new batch of FBS solves my problem.
Thanks again for all your comments,
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