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staining of apoptotic cells

LOCKSHIN, RICHARD A LOCKSHIN at sjumusic.stjohns.edu
Wed Nov 8 08:11:21 EST 1995

contact Luis Benitez at Oncological Research Unit, kAP 107-014,
UNAM, Hosp. 21 Sieglo, Mexico DF 06700, Mexico.  He has some
interesting results along this line.
In article <905kf544 at invrof.anmat.sld.ar> elisa at invrof.anmat.sld.ar ("Dra. Elisa Bal de Kier-Joffe") writes:
>Dear netters:
>We are using Acridine Orange (AO) to stain apoptotic cells.
>The nuclei staining of apoptotic cells is very easily recognized when it is
>in  an advanced stage.
>However when the process is early I observe only a brighter staining of
>chromatin clumps, and a more marked limit in nuclei periphery. When this
>happens, the cytoplasm shows a diffuse red staining which is also evident in
>definite apoptotic cells.
>In contrast normal alive cells only show a pale diffuse green staining of
>the nuclei and cytoplasm and only a kind of red granule (RNA) in the
>cytoplasm next to the nucleus.
>I have not found any comments concerning this early diffuse intense red
>staining of the cytoplasm with AO in apoptotic and preapoptotic cells.
>Could you help me with any ideas, references, etc.?
>Thanks a lot
>(also you can reply to postmaster at invrof.anmat.sld.ar)

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