BSA alternatives for immunoblocking?

Dean Hewish deanh at
Thu Nov 16 21:16:08 EST 1995

We have routinely used casein for blocking. It works better than BSA
but it is not very soluble and is hard to make up. Once dissolved it's
solutions are fairly cloudy but work well for ELISA and blots.

Dean R. Hewish
Cell biologist and Flow Cytometrist
CSIRO Division of Biomolecular Engineering
Parkville, Victoria, Australia

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