DAPI staining of endothelial cells
ras-1 at bones.biochem.ualberta.ca
Tue Nov 21 10:37:19 EST 1995
On 21 Nov 1995, Derrick Grant wrote:
> Does anyone know what's the best way to store stock solutions of
> DAPI, and what is the appropriate concentration to use to stain the
> nucleus of permeabilized endothelial cells in culture.
> Thank you for your help in advance.
We store it as an aqueous solution (1 mg/ml) at -20C. For staining we
normally use at a concentration of 1 ug/ml in PBS.
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