DAPI staining of endothelial cells

Colin Rasmussen ras-1 at bones.biochem.ualberta.ca
Tue Nov 21 10:37:19 EST 1995


On 21 Nov 1995, Derrick Grant wrote:

> Does anyone know what's the best way to store stock solutions of 
> DAPI, and what is the appropriate concentration to use to stain the 
> nucleus of permeabilized endothelial cells in culture.
> 
> Thank you for your help in advance.
> 
We store it as an aqueous solution (1 mg/ml) at -20C.  For staining we 
normally use at a concentration of 1 ug/ml in PBS.

Colin Rasmussen



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