35SMet, 35SCys or 35SCys/Met ?
r-mehta at nimr.mrc.ac.uk
Mon Oct 9 11:48:04 EST 1995
Choice of 35SMet or 35SCys seems to be dependant on Met/Cys content of
the recombinant protein of intrest. However, the 35SMet+35SCysCys mixes
are predominantly used for 'in vivo' labelling of tissue culture cells
as they are a lot cheaper than the purified individual 35S labelled
amino acids. I haven't tested individual 35SMet/Cys mixes - from my
experience Tran35S-label works OK in 'in vivo' labelling expts.
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