HELP: streaking of phosphopeptides on 16% gel

Patrick G. Swann swannp at arb.niams.nih.gov
Wed Oct 18 09:52:49 EST 1995


We're measuring the phosphorylation of purified peptides in an in vitro 
kinase assay using 32P-ATP.  Phosphorylated peptides are separated from 
autophosphorylated kinase by 16% tricine gels.  Lately, we've observed a 
significant streaking (as measured by autoradiogram) in the region of 
the gel at lower molecular weight region of the gel (streaking at 
peptide band and lower; gel looks like a histogram).  Has anyone else 
seen this and does anyone have any advice?

Thanks in advance for your help.





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