HELP: streaking of phosphopeptides on 16% gel
Patrick G. Swann
swannp at arb.niams.nih.gov
Wed Oct 18 09:52:49 EST 1995
We're measuring the phosphorylation of purified peptides in an in vitro
kinase assay using 32P-ATP. Phosphorylated peptides are separated from
autophosphorylated kinase by 16% tricine gels. Lately, we've observed a
significant streaking (as measured by autoradiogram) in the region of
the gel at lower molecular weight region of the gel (streaking at
peptide band and lower; gel looks like a histogram). Has anyone else
seen this and does anyone have any advice?
Thanks in advance for your help.
More information about the Cellbiol