Cell proliferation

Sei Kobayashi kobayasi at MILLES.MED.KYUSHU-U.AC.JP
Mon Sep 4 21:31:02 EST 1995

You may be interested in our recent publication:

Kobayashi et al., Cytosolic Ca2+ transients are not required for
growth factor to induce cell cycle progression of vascular smooth muscle cells 
in primary culture: actions of tyrosine kinase. J. Biol. Chem.
269:9011-9018, 1994

In this study, we developed a fluorescent immunocytochemical method for 
colorimetric determination of cell cycle phase, using monoclonal antibodies 
(anti-PCNA and Ki-67).  The color pattern of the nucleus produced by 
double staining makes feasible differentiation of phases of the cell cycle
at  the level of the single cell.

Sei Kobayashi, M.D., Ph.D.
Division of Molecular Cardiology
Research Institute of Angiocardiology
Faculty of Medicine, Kyushu University, Japan
kobayasi at molcar.med.kyushu-u.ac.jp

At  2:11 PM 95.08.20 +0000, Torsten Boerchers wrote:
>In article <9508091524.AA17118 at ing.ula.ve>, salmen at ING.ULA.VE ("Salmen H.
>>Siham ", Prof. Fac. de Medicina) wrote:
>>>  Hi
>>>  Does anyone could inform me about where to find information on 
>>>  Cell proliferation determination without any radiactive mterials?
>>>  I wold like to substitute for ever the H3 using.
>>>  Thanks
>>>  Siham Salmen M.D
>You may want to look at some alternatives to BrdU, the KI67 antigen,
>which is strongly linked to proliferation:
>Key et al., Assessment of cell proliferation by means of an enzyme-linked 
>immunosorbent assay  based on the detection of the Ki67 protein
>J. Immunol. Meth. 177, (1994) 113-117
>                          Torsten Boerchers                            /

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