Alternative Splicing

jon boyson boyson at primate.wisc.edu
Thu Sep 7 19:53:36 EST 1995


Hi--
I need references and help from people familiar with alternative 
splicing. I've isolated alternatively spliced cDNAs from a MHC class I 
gene which appear fairly "normal," i.e., they are spliced exon to exon. 
However, I've also isolated a fair number of cDNAs from the same prep 
that are spliced in a rather haphazard fashion, e.g., the middle of one 
exon is spliced to the 3'UTR, others have deletions near intron/exon 
boundaries. 

As our lab has little experience in studying splicing, I want to know if 
these are "real," or are they splicing intermediates, or could they be 
experimental artifact (these were all subcloned from RT/PCR reactions 
from total RNA)?  Would we see any difference if we isolate message 
only? Does anyone have an excellent reference on the subject?

Thanks, 
jon





More information about the Cellbiol mailing list