Transfection of normal human cells

Dima Klenchin klenchin at macc.wisc.edu
Sat Sep 9 21:03:10 EST 1995


>I am trying to transfect various "normal" human cell lines with limited 
>success.  I have tried all the standard methodologies.  Does anyone have 
>any suggestions or variations on a theme?  Thanks in advance.

See follow-up to "Efficient L929" transfection" in which I posted
electroporation-related stuff. Worked for me for primary fibroblasts.

- Dima



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