help with lysophosphatidic acid!
Rene Mauricio Salazar
rms1754 at tam2000.tamu.edu
Wed Sep 27 10:22:00 EST 1995
I recently tried to use LPA in cell culture, but I could never get it to
solubilize. The procedure I used was as follows:
1. Dissolve stock LPA (5mg) in a 2:1 mix of chloroform:methanol
2. Add fatty acid-free BSA @ 1% to give a 10 millimolar stock LPA
I would then add this at 100 micromolar to cells cultured in M199. I
have also tried adding calcium and magnesium (.5 mM), but I still got
precipitation using LPA @ a 100 micromolar concentration. If anyone can
offer any advice or can clue me in, please email me. Thanks in advance :^).
Rene' M. Salazar
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