Blunt/sticky ligation

john brennand john.brennand at gbapr.zeneca.com
Tue Apr 2 10:34:47 EST 1996


Jocelyne

Yep no problems with that ligation - its a bog-standard mol. biol 
method.

Do the ligation at 12oC, overnight, as the blunt end ligation is less 
efficient. Also, most of the rapid ligation kits supplied by the major 
manafacturers seem to work well in others hands in our lab.

Use a higher, 10:1, ratio of insert:vector molecules. 





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