adherence of K562 cells

Victor Yankiwski vy05+ at andrew.cmu.edu
Fri Apr 5 12:21:40 EST 1996


Hi,

I'm trying to do SEM of K562 cells, but am having trouble with their
non-adherence to both T.C. dishes and glass cover slips; aspirating
their media before fixation would result in the loss of too many cells. 
Treatment of the plates with collagen does not help much.  Any
suggestions to make them stick, or regarding fixation for SEM would be
appreciated. 

Thanks, 
Victor Yankiwski
vy05 at andrew.cmu.edu 



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